Retention time and peak area
reversed-phase, normal-phase, ion-pair, HILIC, or chiral HPLC
Material Analysis · Chromatography & Diagnostics
High-Performance Liquid Chromatography (HPLC)
Characterise retention time and peak area, purity and component profile, and target concentration with calibration using a scope agreed around your sample and decision.
- Price
- from£15 per sample, excl. VAT
- Turnaround
- 8 working days Preparation and scope dependent
- Available platforms
- Thermo Fisher Vanquish HorizonWaters Alliance iS HPLCShimadzu Nexera X3Agilent 1260
- Typical outputs
- ChromatogramPeak identity/purity tableQuantitative results and system-suitability dataProcessed chromatogram or spectrum
Why this service
What HPLC can help you understand
High-Performance Liquid Chromatography is selected when the project needs defensible information on retention time and peak area, purity and component profile, or target concentration with calibration.…
Purity and component profile
UV/DAD, fluorescence, RI, CAD, or ELSD detection
Target concentration with calibration
UHPLC method development or transfer
Choose the scope
Start from the question, not the tool
The method, preparation route and reporting depth depend on what you need to decide.
Reversed-phase, normal-phase, ion-pair, HILIC, or chiral HPLC
retention time and peak area
- Best used when
- retention time and peak area
- Typical result
- Chromatogram
- Preparation note
- Declare volatility, corrosivity, suspended solids and any storage or temperature requirement.
UV/DAD, fluorescence, RI, CAD, or ELSD detection
purity and component profile
- Best used when
- purity and component profile
- Typical result
- Peak identity/purity table
- Preparation note
- Confirm power, connections, operating state, access restrictions and permitted disassembly.
UHPLC method development or transfer
target concentration with calibration
- Best used when
- target concentration with calibration
- Typical result
- Quantitative results and system-suitability data
- Preparation note
- Confirm dimensions, cutting permission and whether the original geometry must be preserved.
Common outputs
A result package matched to the decision you need to make
Fields, conditions, processing and file formats are confirmed before work begins.
Chromatogram reported with the agreed units, sample reference and measurement conditions.
Peak identity/purity table supplied in a labelled format suitable for direct sample or condition comparison.
Quantitative results and system-suitability data provided with the processing basis and quality checks agreed during technical review.
Processed chromatogram or spectrum included when selected in the confirmed reporting scope.
Sample requirements
What to provide before work begins
Provide representative, clearly labelled samples and identify the decision, feature or comparison that matters.
| Suitable sample | Submission requirement | Planning note |
|---|---|---|
| Liquid / solution | Provide a sealed, compatible container labelled with composition, concentration and solvent. | Declare volatility, corrosivity, suspended solids and any storage or temperature requirement. |
| Device / assembly | Provide a complete labelled device or assembly with drawings, interfaces and operating limits. | Confirm power, connections, operating state, access restrictions and permitted disassembly. |
| Solid / component | Provide a clean, stable and clearly labelled specimen with the target face or region identified. | Confirm dimensions, cutting permission and whether the original geometry must be preserved. |
Objective: state the decision, comparison or acceptance criterion the work must support.
Handling and access: declare hazards, instability, confidentiality, file constraints or special logistics before dispatch or transfer.
Questions and answers
Common planning decisions
Short answers to issues that can change preparation, scope, timing or interpretation.
What should I send for High-Performance Liquid Chromatography?
Provide liquid / solution, device / assembly, solid / component as applicable. Exact quantity, dimensions and preparation are confirmed during technical review.
Which analysis option should I choose?
The final method, preparation and reporting scope are confirmed after the sample and project objective have been reviewed.
How long will the test take?
Typical turnaround is 8 working days after sample acceptance and method confirmation.
What can limit the result?
A detector responds only to compatible compounds. Co-elution and sample instability require method development or orthogonal confirmation.
Configure the service
Send the information needed to scope HPLC correctly
- Sample form, material, dimensions and quantity
- Feature or decision the result must address
- Required comparison, acceptance criterion or reference
- Preferred output and reporting depth