Fluorescence intensity and spatial distribution
wide-field or confocal fluorescence imaging
Material Analysis · Microscopy & Imaging
Fluorescence Microscopy (FM)
Characterise fluorescence intensity and spatial distribution, co-localisation, feature size, and area fraction, and time-, wavelength-, or condition-dependent emission where configured using a scope agreed around your sample and…
- Price
- from£30 per sample, excl. VAT
- Turnaround
- 7 working days Preparation and scope dependent
- Available platforms
- ZEISS LSM 990Evident FLUOVIEW FV4000Olympus BX51
- Typical outputs
- Multichannel fluorescence micrographsIntensity profiles, co-localisation, or area statisticsAcquisition settings and representative raw image filesMethod-specific images
Why this test
What FM can help you understand
Fluorescence Microscopy is selected when the project needs defensible information on fluorescence intensity and spatial distribution, co-localisation, feature size, and area fraction, or time-, wavelength-, or…
Co-localisation, feature size, and area fraction
spectral or z-stack acquisition
Time-, wavelength-, or condition-dependent emission where configured
time-lapse or quantitative image analysis
Choose the scope
Start from the question, not the instrument
The measurement mode, preparation route and reporting depth depend on what you need to learn from the sample.
Wide-field or confocal fluorescence imaging
fluorescence intensity and spatial distribution
- Best used when
- fluorescence intensity and spatial distribution
- Typical result
- Multichannel fluorescence micrographs
- Preparation note
- Exact mass and any drying or dispersion step are confirmed after technical review.
Spectral or z-stack acquisition
co-localisation, feature size, and area fraction
- Best used when
- co-localisation, feature size, and area fraction
- Typical result
- Intensity profiles, co-localisation, or area statistics
- Preparation note
- State the substrate, nominal thickness and whether sectioning or mounting is permitted.
Time-lapse or quantitative image analysis
time-, wavelength-, or condition-dependent emission where configured
- Best used when
- time-, wavelength-, or condition-dependent emission where configured
- Typical result
- Acquisition settings and representative raw image files
- Preparation note
- Declare biological hazards and confirm fixation, dehydration, temperature and disposal requirements.
Common outputs
A result package matched to the decision you need to make
Fields, measurement conditions, processing and file formats are confirmed before work begins.
Multichannel fluorescence micrographs reported with the agreed units, sample reference and measurement conditions.
Intensity profiles, co-localisation, or area statistics supplied in a labelled format suitable for direct sample or condition comparison.
Acquisition settings and representative raw image files provided with the processing basis and quality checks agreed during technical review.
Method-specific images included when selected in the confirmed reporting scope.
Sample requirements
What to send
Provide representative, clearly labelled samples and identify the region, feature or comparison that matters.
| Suitable sample or input | Submission requirement | Planning note |
|---|---|---|
| Powder / particles | Provide a dry, homogeneous and clearly labelled sample in a sealed container. | Exact mass and any drying or dispersion step are confirmed after technical review. |
| Film / coating | Provide the coated substrate or a flat film with the test surface and coating side clearly marked. | State the substrate, nominal thickness and whether sectioning or mounting is permitted. |
| Biological / soft material | Provide fixed, preserved or otherwise stabilised material in the agreed container and medium. | Declare biological hazards and confirm fixation, dehydration, temperature and disposal requirements. |
| Test coupon / specimen | Provide labelled specimens prepared to the agreed geometry, orientation and surface condition. | Confirm the applicable standard, dimensions, loading direction and number of replicates. |
Region of interest: include a marked image, sketch or reference when the target feature is localised.
Handling: declare hazardous, magnetic, volatile, moisture-sensitive, beam-sensitive or temperature-controlled material before dispatch.
Questions and answers
Common planning decisions
Short answers to issues that can change preparation, scope or interpretation.
What should I send for Fluorescence Microscopy?
Provide powder / particles, film / coating, biological / soft material, test coupon / specimen as applicable. Exact quantity, dimensions and preparation are confirmed during technical review.
Which analysis option should I choose?
The final method, preparation and reporting scope are confirmed after the sample and project objective have been reviewed.
How long will the test take?
Typical turnaround is 7 working days after sample acceptance and method confirmation.
What can limit the result?
Photobleaching, autofluorescence, spectral bleed-through, and labelling efficiency can bias interpretation. The label reports the selected target and may not represent the full sample composition.
Configure the service
Send the information needed to scope FM correctly
- Sample form, material, dimensions and quantity
- Feature or decision the result must address
- Marked region of interest where relevant
- Required comparison, output and reporting depth